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**Mass spectrometry** (MS) is, without a doubt, a workhorse technique for isotopologue analysis. This method measures the mass-to-charge ratio of ions. Since isotopologues have different masses, MS can easily distinguish between them. There are several flavors of MS, including gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and isotope ratio mass spectrometry (IRMS). IRMS is particularly useful for measuring the relative abundance of different isotopes in a sample with high precision. Scientists use this data to calculate the ratios of different isotopes, which can reveal valuable information about a sample's origin or the processes it has undergone. IRMS is often used in environmental science, geochemistry, and food authentication because of its ability to analyze tiny differences in isotopic composition.
